Research on the enhancement material and culture method of soil aggregates composed of feldspathic sandstone and sand

The Mu Us Sandy Land is a region characterized by wind-blown sand and soil erosion in northern China. To enhance the soil quality of this area, various organic materials were incorporated into the mixed soil at a volume ratio of 1:2 for feldspathic sandstone to sand. Culture was conducted in the field and under constant temperature conditions in laboratory culture chambers. Four treatments were established in the experiment, each calculated based on weight ratio and controlled (with no organic material added, CK); single application of straw (5% straw, P1); single application of biochar (5% biochar, P2); combined application of biochar and straw (5% biochar + 5% straw, P3). After 90 days of culture, soil samples were collected for analysis of various indicators such as soil aggregate particle size distribution, water stability of soil aggregates, mean weight diameter, mean geometric diameter, and fractal dimension using dry sieving and wet sieving methods. The objective is to establish a scientific basis and provide technical support for addressing the challenges associated with compound soil and implementing rational fertilization measures. The research results indicate that: (1) The quantity of aggregates > 0.25 mm under different treatments follows the order CK < P1 < P2 < P3, and the differences between treatments are significant (P < 0.05); (2) Soil water stability, mean weight diameter (MWD), mean geometric diameter (GMD), and fractal dimension of soil aggregates in compound soil with different organic material additions are superior to the control, and the effect of biochar on improving soil aggregates is better than that of corn straw. The combined application of both significantly improves the effect compared to single applications. In both culture modes, under wet sieving, the P3 treatment shows the highest MWD and GMD of soil aggregates, with an increase ranging from 3.45% to 85% and 4.55% to 38.46%, respectively, compared to other treatments. (3) The trend of fractal dimension among treatments is consistent: P3 < P2 < P1 < CK, and the differences between treatments are significant (P < 0.05). Moreover, there is a good negative correlation linear equation relationship between the fractal dimension (y) and WR > 0.25 (x) of compound soil, with a correlation coefficient of up to 0.9851. In conclusion, the incorporation of organic materials can effectively enhance the proportion of macroaggregates in compound soil consisting of Feldspathic sandstone and sand, thereby improving soil stability and erosion resistance. The optimal outcome is achieved through the combined application of biochar and straw. Indoor culture proves to be more effective than field culture, while wet sieving accurately reflects the structural characteristics of compound soil under both dry and wet sieving treatments.


Experimental design
The experiment was divided into indoor and field culture experiments, with four treatments established.The original Feldspathic sandstone to sand volume ratio was 1:2.The control group (CK) used composite soil without any organic materials.Organic materials, specifically corn straw and biological carbon, were added at a 5% volume ratio after being passed through a 1 mm screen (Table 1).An indoor culture experiment was conducted with test materials weighing 2500 g in each test pot, including soil samples and added materials.Five repetitions were set, with 70% distilled water of field water capacity added to each treated sample.The pots were completely sealed with plastic and had 5 reserved gas exchange ports arranged in a plum shape.Using a weighing method daily, the moisture lost by evaporation was replenished promptly to maintain soil moisture content within the field water capacity range.In the field culture experiment, conducted at the Qinling Field Monitoring Central Station in Meixian County, Baoji, Shaanxi Province, with a plot area of 25m 2 , each treatment was repeated three times.Biological carbon was applied before the experiment began, spread evenly on the surface, and manually cultivated into the 0-20 cm soil layer.Straw was applied through shallow rotation, with ploughing depths of 0-20 cm.

Sample collection
The experiment commenced on March 1, 2023, and concluded on July 1, spanning a duration of 3 months.Following the experiment, soil samples from the pot were collected using the quartering method, with the weight of the samples representing half of the total weight.The soil was then fragmented along the natural cracks until reaching approximately 10 mm in size.Subsequently, the soil was air-dried naturally, sieved through screens with particle sizes of 5 mm and 2 mm, and a 200 g mixed sample was weighed based on the proportion of particle sizes.

Determination indicators and methods
The particle size distribution and stability of soil aggregates are assessed through dry sieving and wet sieving methods [24][25][26] .Dry sieving involves separating soil samples into different particle size fractions using an oscillating sieve shaker to determine aggregate stability at each size grade.Wet sieving employs an aggregate analyzer to measure stability, with water-stable aggregates at each particle size being washed into aluminum containers using distilled water, followed by drying and weighing for mass calculation.

Data analysis
Soil aggregate parameters.Typically, parameters such as the percentage content of soil aggregates > 0.25 mm (R 0.25 ), water-stable aggregate stability rate (WASR), mean weight diameter (MWD), mean geometric diameter (GWD), and fractal dimension (D) are selected for calculation and explanation 27 .The calculation formulas are as follows: (1)   x i represents the mean diameter of the aggregate at the i-th particle size grade.W i denotes the proportion of the weight of aggregates at the i-th particle size grade; W i signifies the weight of aggregates at different particle size grades in the composite soil.DR 0.25 /WR 0.25 stands for the percentage content of > 0.25 mm force-stable/waterstable aggregates (%); M(r < x i ) represents the weight of aggregates with a particle size smaller than r; that is, the maximum particle size of the aggregates.Data analysis was conducted using Excel 2022 for initial processing, followed by statistical analysis of the data using Spss software.Multiple comparisons were performed using LSD (Least Significant Difference).

Composite soil aggregates
The impact of soil aggregate structure on soil fertility and stability is a topic of increasing research interest 28,29 .Soil aggregates play a crucial role in enhancing soil aeration, water retention, and nutrient adsorption.Different types of aggregates have varying abilities in these functions 30 .As illustrated in Fig. 3, both field and laboratory studies have shown that the composition of aggregates of varying particle sizes in composite soils with different additives exhibit a consistent trend under dry and wet sieving treatments.Specifically, the content of aggregates larger than 0.25 mm follows the order: CK < P1 < P2 < P3, with statistically significant differences observed between the treatments (P < 0.05).Under the field culture treatment P3, there is a higher content of aggregates > 0.25 mm observed under both dry and wet sieving treatments when compared to other treatments.Specifically, the content is higher by 16.98%, 17.01%, 10.08% and 21.05%, 8.45%, 2.15%, respectively.Similarly, under the experimental culture, the content is higher by 30.36%, 12.83%, 8.19% and 11.98%, 9.12%, 0.82%, respectively.A comprehensive analysis suggests that the addition of straw and biochar promotes the formation of soil aggregate structure, leading to a significant increase in the formation of large aggregates.

Water stability of aggregates
The impact of various organic materials and their quantities on the water stability of composite soil aggregates is shown to vary (Fig. 4), with significant differences observed between treatments (P < 0.05).Across different cultivation methods, a consistent trend is observed in the water stability of aggregates with a content > 0.25 mm, where the sequence from lowest to highest is CK < P1 < P2 < P3, with significant differences between treatments (P < 0.05).In both field and culture experiments, the water stability of aggregates under treatments P1, P2, and P3 is higher compared to the control CK.The percentages range from 1.52%, 6.07%, and 19.95% to 26.13%, 39.37%, and 48.64%, respectively.Treatment P3 demonstrates the highest stability, showing an increase ranging from 13.09% to 19.95% and 6.65% to 48.64% when compared to other treatments.A comparative analysis indicates that the use of materials can enhance the stability of composite soil, with biochar application showing superior results to straw application alone.Moreover, when combined, the stability of composite soil is notably enhanced.
Soil aggregate mean weight diameter (MWD) and mean geometric diameter (GMD) are among the evaluation indicators of soil structure 25,26 .These parameters primarily reflect the size and distribution arrangement of soil aggregates.Higher values of MWD and GMD indicate a more significant soil aggregation effect, suggesting stability and maturity in development stages.They also play a role in promoting soil water retention and fertility to some extent 31 .Under dry and wet sieving methods, the MWD and GMD of composite soils under different treatments are shown in Fig. 5.The performance of soil stability indicators under different treatments in dry and wet sieving methods is consistent with that of water stability of aggregates.The results show that the effects of organic material addition are better than untreated controls, and the improvement effects of composite organic materials on composite soil are better than single organic materials.Under both field and laboratory culture experiments, treatment P3 exhibits optimal performance in MWD and GMD under dry and wet sieving methods, with no significant differences observed compared to the control CK treatment (P < 0.05).In field and laboratory culture experiments, when comparing treatment P3 to other treatments using dry and wet sieving methods, the increase in MWD and GMD of composite soil ranges from 11.11% to 50.00%, 9.68% to 25.93%, 3.57% to 20.83%, 4.76% to 15.79%, and 15.63% to 85.00%, 12.50% to 38.46%, 3.45% to 30.43%, 4.55% to 27.78%, respectively.Comparative analysis under different culture conditions shows that in laboratory culture, the MWD and GMD of composite soil treated with P3 are better than those in field culture experiments using dry and wet sieving x i x max methods.There were increases of 23.33%, 5.88%, 3.45%, and 4.55%, respectively, with no significant differences between treatments (P < 0.05).

Fractal dimension
The fractal dimension is a more precise indicator that reflects the characteristics of soil aggregates.By using the equation for determining soil particle size-weight distribution, the fractal equation for particle size distribution can be directly calculated, enabling the accurate and direct computation of soil particle fractal dimensions.This measurement primarily captures variations in soil particle sizes and, to some extent, the level of uniformity in texture distribution.In general, a higher fractal dimension indicates lower stability, and conversely 32,33 .The fractal dimension of soil is negatively correlated with the stability of soil aggregates (Table 2).The influence of different additives and their quantities on the fractal dimension of composite soil is shown in Fig. 6.Under dry sieving, the fractal dimension distribution ranges from 2.79 to 2.85 for field and experimental cultures, while under wet sieving, the range is 2.85 to 2.90 and 2.85 to 2.91, respectively.The trend of fractal dimension across treatments shows P3 < P2 < P1 < CK consistently, with significant differences between treatments (P < 0.05).Treatment P3 demonstrates the most notable enhancement in the composite soil structure.

Effects of different materials on the composition of composite soil aggregates
Soil aggregates are discontinuous structural units formed by natural or anthropogenic processes, including clods, aggregates, and other units.Several studies have demonstrated that various organic materials, like biochar and straw incorporation, can significantly enhance soil organic carbon content and encourage the development of larger soil aggregates 10,11 .Biochar typically has a high specific surface area and contains numerous organic molecules, both of which can effectively facilitate the formation of large aggregates 34 .Long-term field experiments on straw incorporation into loess soil have indicated no significant improvement in soil aggregate composition, but there is an observed increasing trend in the content of large aggregates in the soil 35 .The analysis of Haplic Luvisols with straw and biochar application reveals that organic materials can greatly enhance the presence of large aggregates in the soil.However, the increase in advantageous particle size differs depending on the type of material added.For instance, straw incorporation significantly increases the content of aggregates > 1 mm, while the combined use of biochar and straw is conducive to the formation of aggregates > 0.25 mm 36 .This is consistent with the results of this study, where the content of aggregates > 0.25 mm follows the sequence CK < P1 < P2 < P3, and the differences between treatments are significant (P < 0.05).The addition of organic materials is advantageous for enhancing the structure of composite soil, with biochar demonstrating a greater impact compared to straw.This is primarily due to biochar's extensive specific surface area and substantial negative charge on its surface, which enhances the exchange capacity of soil cations and facilitates the formation of larger soil aggregates more efficiently than straw.However, the optimal effect is achieved through the combined application of both materials 37 .Specifically, under field culture, treatment P3 shows higher content of

Effects of different materials on the stability and size of composite soil aggregates
The WASR of composite soil is a reliable indicator of soil structure stability, where a higher value signifies greater stability.Meanwhile, the MWD and GMD of soil aggregates can provide insights into the particle size distribution characteristics of aggregates following various tillage treatments.MWD and GMD are commonly utilized as metrics to assess soil aggregate condition, with larger values suggesting improved aggregation and enhanced stability of soil aggregates 37 .Under various cultural practices, the WASR of composite soil displays a consistent pattern across different treatments, aligning with the distribution of aggregates > 0.25 mm in size, following the sequence CK < P1 < P2 < P3.The observed differences between treatments are statistically significant (P < 0.05).Previous research has indicated that the incorporation of wheat straw ash biochar into red soil resulted in a 28% increase in MWD of the soil, while the addition of herbaceous plant biochar to clay soil led to a 21% increase in MWD of soil aggregates 38,39 .The study found that both MWD and GMD exhibited a sequence of CK < P1 < P2 < P3 under different treatments.When dry sieving was used, the MWD and GMD increased by 25.00% to 50.00%, 11.11% to 25.93%, and 50.00% to 85.00%, 11.54% to 38.46% under field and laboratory conditions for each treatment compared to the control CK.Similarly, under wet sieving, the increases ranged from 12.50% to 20.83%, 5.26% to 15.79%, and 8.69% to 30.43%, 5.56% to 27.78%, respectively.The differences between treatments were found to be statistically significant (P < 0.05).Under both culture conditions, treatment P3 exhibits the highest MWD and GMD of aggregates, with increases compared to other treatments ranging from 3.45% ~ 85.00% and 4.55% ~ 38.46%, respectively.

Fractal dimension of composite soil and its correlation analysis
Fractal dimension is a crucial indicator for quantitatively assessing soil structure stability.It can reflect the uniformity of texture in aggregates and provide insights into properties such as soil water, nutrients, air, and temperature.The expression of fractal dimension is meaningful and functional, enabling a comprehensive and rational representation of soil properties and functions 40 .Studies on straw incorporation have shown that the fractal dimension can indicate aggregate distribution and respond to changes in soil aggregates at varying straw incorporation rates.The fractal dimension is inversely related to the mean weight diameter and geometric mean diameter, suggesting that higher fractal dimension values are associated with less stable soil structures, while lower values are linked to more stable structures 41,42 .The results of this study reveal a consistent trend in the fractal dimension across treatments, with the order P3 < P2 < P1 < CK, and statistically significant differences between treatments (P < 0.05).Additionally, a strong negative linear correlation is observed between the fractal dimension of composite soil (y) and the content of aggregates > 0.25 mm (x), with a high correlation coefficient of 0.9851.

Conclusion
(1) A comprehensive analysis of soil aggregate stability parameters, such as the content of aggregates > 0.25 mm, mean weight diameter (MWD), geometric mean diameter (GMD), fractal dimension (D), and waterstable aggregate stability rate under dry sieving and wet sieving methods, revealed that the incorporation of organic materials enhances soil stability.This enhancement is evidenced by the significant increase in large soil aggregates, leading to an overall improvement and enhancement of soil structure.(2) Analysis of soil aggregate stability parameters under the addition of single organic material and mixed organic material, the improvement achieved with mixed materials is superior to that with single material, and the improvement effect is significant.(3) The impact of field culture and laboratory culture on the stability of composite soil with varying organic material additions was analyzed.Both culture methods were found to enhance the structure and stability of composite soil, yielding positive outcomes.Laboratory culture demonstrated better results than field culture.When comparing the characteristics of composite soil aggregates under dry sieving and wet sieving treatments, it was observed that wet sieving resulted in smaller data variability, providing a more accurate reflection of the structural characteristics of composite soil.
The structural stability of aeolian sand soil can be enhanced by incorporating biological straw and biochar.Subsequent experimental studies will focus on refining the structural properties of aeolian sand soil, with a particular emphasis on identifying the most effective material type and dosage.By systematically varying the type and quantity of added materials, this research aims to establish a sound scientific foundation for enhancing the structural integrity and overall quality of sandy land.

Figure 1 .
Figure 1.Location of the test site.

Figure 2 .
Figure 2. Sand, arsenic sandstone, and compounded soil images and SEM images.

Figure 3 .Figure 4 .
Figure 3. Percentage content of aggregates with different granule diameters under different additives was analyzed.

Figure 5 .
Figure 5.Effect of different organic material types and amount on the stability parameters of the compound soil.* indicated significant difference between treatments (P < 0.05); ** indicated significant difference between treatments (P < 0.01).The same below.
www.nature.com/scientificreports/RelationshipanalysisA higher percentage of aggregates > 0.25 mm is advantageous for creating a well-aggregated structure and enhancing the soil's ability to withstand external forces.The relationship between the fractal dimension of composite soil and the percentage content of aggregates > 0.25 mm under different treatments is shown in Fig.5.Analysis demonstrates a strong linear relationship and a notable negative correlation between the fractal dimension of composite soil aggregates and the percentage content of aggregates > 0.25 mm.Under dry sieving, the relationship between the fractal dimension (y) and WR > 0.25 (x) under field and experimental culture conditions is y = -0.0042x+ 3.0743 (R 2 = 0.9461) and y = -0.0033x+ 3.0095 (R 2 = 0.843), respectively.Under wet sieving, the relationships are y = -0.0034x+ 3.0136 (R 2 = 0.7593) and y = -0.0043x+ 3.0419 (R 2 = 0.9851), respectively.The regression equations suggest that as the content of aggregates > 0.25 mm increases in composite soil, the soil structure and aggregation become more stable, resulting in enhanced erosion resistance.Conversely, a lower fractal dimension indicates a more mature stage of soil development.

Table 2 .
Effect of different organic material types and amount on the fractal dimension of composite soil.